3d volumetric reconstruction  (Oxford Instruments)

Journal: Fluids and Barriers of the CNS

Article Title: Ablation of the choroid plexus attenuates hydrocephalus-induced parenchymal edema with moderate reduction of postnatal hippocampal neurogenesis

doi: 10.1186/s12987-026-00882-7

Figure Lengend Snippet: ROSA26-iDTR model does not efficiently ablate ChP or reduce CSF volume in neonates before P10. ( A ) Experimental timeline and description of the four groups of mice (genotype and Dtx treatment dosage/timing of treatment). ( B ) Representative 3D Imaris-reconstructed ventricular volume in the four groups of mice. ( C ) Quantification of individual ventricles and total ventricular volume. Mean+SEM. Each data point is the data from an individual animal. Circle = female and Triangle = male. p- value as indicated, one-way ANOVA for each ventricle for panel C

Article Snippet: Fig. 2 ROSA26-iDTR model does not efficiently ablate ChP or reduce CSF volume in neonates before P10. ( A ) Experimental timeline and description of the four groups of mice (genotype and Dtx treatment dosage/timing of treatment). ( B ) Representative 3D Imaris-reconstructed ventricular volume in the four groups of mice. ( C ) Quantification of individual ventricles and total ventricular volume.

Techniques:

Journal: EMBO Reports

Article Title: Vimentin loss inhibits DNA damage responses and promotes cancer cell survival

doi: 10.1038/s44319-026-00913-2

Figure Lengend Snippet: ( A ) (Left) Representative maximum intensity projection of confocal z-stacks of U251-MG cells plated on glass coverslips and stained for vimentin, a-tubulin and DAPI. (Right) Inset of inverted LUT of boxed region showing vimentin staining around the nucleus (scale bar 10 µm). ( B ) Representative maximum intensity projection of confocal images of NLS-mCherry control or Vim KO U251-MG cells plated on glass coverslips and stained for SUN1 and DAPI (scale bar 10 µm). ( C ) SuperPlots of nuclear aspect ratio. Individual data points are represented as small dots in grey (Ctrl) or blue (Vim KO). The mean of each repeat is shown by bigger dots. Data were acquired from four independent experiments and analysed using a two-tailed t -test. Error bars represent mean ± SEM. ( D ) Representative orthogonal view or z-stacks acquired by confocal microscopy of control and Vim KO U251-MG cells. ( E ) SuperPlots of nuclear aspect ratio. Individual data points are represented as small dots in grey (Ctrl) or blue (Vim KO). The mean of each repeat is shown by bigger dots. Data were acquired from three independent experiments and analysed using a two-tailed t -test. Error bars represent mean ± SEM. ( F ) Representative scheme of the cell confiner device. ( G ) SuperPlots of migration speed of control or Vim KO under no, medium (~3.5 µm) or high (~2 µm) compression (Movies and ). Individual data points are represented as small dots. The mean of each repeat is shown by bigger dots. Data were acquired from three independent experiments and analysed using a one-way ANOVA test. Error bars represent mean ± SEM. ( H ) Schematic of the microplate rheology used to deform a cell in suspension from its original size to 3 µm thickness and measure the ( I ) maximal force and ( J ) stress needed for this deformation. Data were acquired from three independent experiments and analysed using a two-tailed t -test. Error bars represent mean ± SEM. ( K ) Expression of lamin A/C in control, Vim KO U251-MG cell line and in human astrocytes was analysed by western blot and quantified using Fiji. Data were acquired from three independent experiments and analysed using the Kruskal–Wallis test. Error bars represent mean ± SEM. ( L ) SuperPlots of nuclear height in Ctrl or Vim KO U251-MG cells stably expressing KASHext (left) or DN-KASH (right). Individual data points are shown as small dots in grey (Ctrl) or blue (Vim KO); the mean of each repeat is indicated by larger dots. Data were acquired from three independent experiments and analysed by one-way ANOVA. Error bars represent mean ± SEM. ( M ) Representative 3D reconstructions generated using IMARIS software from confocal z-stacks of control and Vim KO U251-MG cells plated on glass coverslips and stained for α-tubulin and DAPI (Left) or F-actin and DAPI (Right) (scale bar: 8 µm). ( N ) SuperPlots of the ratio of microtubule intensity between the nucleus and the cytoplasm. Individual data points are represented as small dots in grey (Ctrl) or blue (Vim KO). The mean of each repeat is shown by bigger dots. Data were acquired from four independent experiments and analysed using a two-tailed t -test. Error bars represent mean ± SEM. ( O ) SuperPlots of the ratio of actin intensity between the nucleus and the cytoplasm. Individual data points are represented as small dots in grey (Ctrl) or blue (Vim KO). The mean of each repeat is shown by bigger dots. Data were acquired from four independent experiments and analysed using a two-tailed t -test. Error bars represent mean ± SEM. ( P ) SuperPlots of nuclear height in Ctrl or Vim KO U251-MG cells treated for 24 h with DMSO (left) or Nocodazole (NOCO, right). Individual data points are shown as small dots in grey (Ctrl) or blue (Vim KO); the mean of each repeat is indicated by larger dots. Data were acquired from three independent experiments and analysed by one-way ANOVA. Error bars represent mean ± SEM. ( Q ) SuperPlots of nuclear aspect ratio in Ctrl or Vim KO U251-MG cells treated for 24 h with DMSO (left) or Nocodazole (right). Individual data points are shown as small dots in grey (Ctrl) or blue (Vim KO); the mean of each repeat is indicated by larger dots. Data were acquired from three independent experiments and analysed by one-way ANOVA. Error bars represent mean ± SEM.

Article Snippet: Error bars represent mean ± SEM. ( M ) Representative 3D reconstructions generated using IMARIS software from confocal z-stacks of control and Vim KO U251-MG cells plated on glass coverslips and stained for α-tubulin and DAPI (Left) or F-actin and DAPI (Right) (scale bar: 8 μm). ( N ) SuperPlots of the ratio of microtubule intensity between the nucleus and the cytoplasm.

Techniques: Confocal, Glass, Staining, Control, Two Tailed Test, Microscopy, Migration, Suspension, Expressing, Western Blot, Stable Transfection, Generated, Software